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Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing.

Chen Y, Hu Y, Wang X, Luo S, Yang N, Chen Y, Li Z, Zhou Q, Li W
Innovation (Camb). 2022 May 26;3(4):100264. doi: 10.1016/j.xinn.2022.100264. eCollection 2022 Jul 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188268pCAG-Cas12i-Max-T2A-EGFPMammalian expression, Genome editing
188269pCAG-Cas12i-HiFi-T2A-EGFPMammalian expression, Genome editing
188270pCAG-Cas12i2-T2A-EGFPMammalian expression, Genome editing
188271pCAG-AaCas12b-T2A-EGFPMammalian expression, Genome editing
188272pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFPMammalian expression, Genome editing
188273pCAG-PlmCasX-T2A-EGFPMammalian expression, Genome editing
188274pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFPMammalian expression, Genome editing
188275BPK2014-Cas12i2prokaryotic expression
188276BPK2014-Cas12i-Maxprokaryotic expression

Antibodies from Article