Use of orthogonal serine integrases to multiplex plasmid conjugation and integration from E. coli into Streptomyces.
Gao H, Smith MCM
Access Microbiol. 2021 Dec 8;3(12):000291. doi: 10.1099/acmi.0.000291. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188253 | pHG5 | Integrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selection |
| 188254 | pHG6 | Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection |
| 188255 | pHG7 | Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection |