Chemically inducible split protein regulators for mammalian cells.
Rihtar E, Lebar T, Lainscek D, Kores K, Lesnik S, Bren U, Jerala R
Nat Chem Biol. 2022 Sep 26. doi: 10.1038/s41589-022-01136-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188256 | dCas9-NLS-gs10-cDHFR174 | Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus |
| 188257 | dCas9-NLS-gs10-cERb447 | Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus |
| 188258 | dCas9-NLS-gs10-cGR2176 | Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus |
| 188259 | dCas9-NLS-gs10-cLYN268 | Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus |
| 188261 | dCas9-NLS-gs10-cTRb413 | Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus |
| 188262 | nDHFR173-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |
| 188263 | nERb446-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |
| 188264 | nGR2175-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |
| 188265 | nLYN267-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |
| 188266 | nPPARg429-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |
| 188267 | nTRb412-gs10-NLS-VPR | Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus |