Addgene: Chemically inducible split protein regulators for mammalian cells.

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Roman Jerala
Rihtar et al

Chemically inducible split protein regulators for mammalian cells.

Rihtar E, Lebar T, Lainscek D, Kores K, Lesnik S, Bren U, Jerala R
Nat Chem Biol. 2022 Sep 26. doi: 10.1038/s41589-022-01136-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
188267	nTRb412-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188266	nPPARg429-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188265	nLYN267-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188264	nGR2175-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188263	nERb446-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188262	nDHFR173-gs10-NLS-VPR	Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
188261	dCas9-NLS-gs10-cTRb413	Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
188259	dCas9-NLS-gs10-cLYN268	Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
188258	dCas9-NLS-gs10-cGR2176	Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
188257	dCas9-NLS-gs10-cERb447	Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

Antibodies from Article