Engineering circular RNA for enhanced protein production.
Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen CK, Wender PA, Chang HY
Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 10.1038/s41587-022-01393-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188115 | circRNA-synIRES-R25-mNeonGreen | Template plasmid to transcribe circular RNA encoding mNeonGreen |
| 188116 | circRNA-synIRES-R25-NanoLuc | Template plasmid to transcribe circular RNA encoding NanoLuc |
| 188247 | mRNA-CleanCap-NanoLuc | Template plasmid to transcribe mRNA encoding NanoLuc |
| 188248 | mRNA-CleanCap-mNeonGreen | Template plasmid to transcribe mRNA encoding mNeonGreen |
| 188249 | mRNA-CleanCap entry vector | Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion |
| 189966 | pRC0569 (parts entry vector) | Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion |
| 189967 | pRC0940 (circRNA entry vector) | Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion |
| 189968 | CVB3 IRES | Part 2 plasmid to introduce the CVB3 IRES into circRNAs |
| 189969 | HRV-B3 IRES | Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs |
| 189970 | synIRES_RC25 | Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs |
| 189972 | T4 td upstream intron-PABP | Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs |
| 189974 | HBA1 full 3'UTR-T4 td downstream intron | Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs |
| 189975 | 3xFLAG-NanoLuc | Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs |
| 189976 | mNeonGreen | Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs |