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Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation.

Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. Epub 2023 Feb 15. (Link opens in a new window) PubMed (Link opens in a new window) Article


Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:

  • James Hurley
  • Michael Lazarou

    • Plasmids from Article

    ID Plasmid Purpose
    188073pCAG-GST-ATG9 (828-839)-ATG101 (1-198)To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells
    188074pCAG-ATG13 (1-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
    188075pCAG-OSF-ATG13 (2-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
    188076pCAG-MBP-ATG9Recombinant overexpression of human ATG9 in mammalian cells
    188077pCAG-MBP-ATG9-V839AExpresses human ATG9-V839A mutant in mammalian cells
    188078pCAG-MBP-ATG9-K838AExpresses human ATG9-K838A mutant in mammalian cells
    188079pCAG-MBP-ATG9-H837AExpresses human ATG9-H837A mutant in mammalian cells
    188080pCAG-MBP-ATG9-V836AExpresses human ATG9-V836A mutant in mammalian cells
    188081pCAG-MBP-ATG9-Q835AExpresses human ATG9-Q835A mutant in mammalian cells
    188082pCAG-MBP-ATG9-P834AExpresses human ATG9-P834A mutant in mammalian cells
    188083pCAG-MBP-ATG9-P833AExpresses human ATG9-P833A mutant in mammalian cells
    188084pCAG-MBP-ATG9-L832AExpresses human ATG9-L832A mutant in mammalian cells
    188085pCAG-MBP-ATG9-D830A/E831AExpresses human ATG9-D830A/E831A mutant in mammalian cells
    188086pCAG-MBP-ATG9 (1-830)Expresses human ATG9 C-terminal truncation mutant in mammalian cells
    188087pCAG-MBP-Foldon-ATG9 (692-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
    188088pCAG-MBP-Foldon-ATG9 (723-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
    188089pCAG-MBP-Foldon-ATG9 (801-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
    188090pCAG-MBP-Foldon-ATG9 (828-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
    188091pCAG-MBP-Foldon-ATG9 (830-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
    188092pCAG-MBP-ATG9 (692-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
    188093pCAG-MBP-ATG9 (801-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
    188094pCAG-OSF-ATG13 (2-197)-K15DExpresses human ATG13 HORMA K15D mutant in mammalian cells
    188095pCAG-OSF-ATG13 (2-197)-F16DExpresses human ATG13 HORMA F16D mutant in mammalian cells
    188096pCAG-OSF-ATG13 (2-197)-K18DExpresses human ATG13 HORMA K18D mutant in mammalian cells
    188097pCAG-OSF-ATG13 (2-197)-F19DExpresses human ATG13 HORMA F19D mutant in mammalian cells
    188098pCAG-OSF-ATG13 (2-197)-W50DExpresses human ATG13 HORMA W50D mutant in mammalian cells
    188099pCAG-OSF-ATG13 (2-197)-E83LExpresses human ATG13 HORMA E83L mutant in mammalian cells
    188100pCAG-OSF-ATG13 (2-197)-Y115DExpresses human ATG13 HORMA Y115D mutant in mammalian cells
    188101pCAG-OSF-ATG13 (2-197)-Y118DExpresses human ATG13 HORMA Y118D mutant in mammalian cells
    188102pCAG-OSF-ATG13 (2-197)-L151DExpresses human ATG13 HORMA L151D mutant in mammalian cells
    188103pCAG-OSF-ATG13 (2-197)-Y152DExpresses human ATG13 HORMA Y152D mutant in mammalian cells
    188104pCAG-OSF-ATG13 (2-197)-R153DExpresses human ATG13 HORMA R153D mutant in mammalian cells
    188105pCAG-GST-ATG101-Y45DExpresses human ATG101 HORMA Y45D mutant in mammalian cells
    188106pCAG-GST-ATG101-W110D/F112DExpresses human ATG101 HORMA W110D/F112D mutant in mammalian cells
    188638pBMN HA-ATG13(Y118D)For expression of human ATG13(Y118D)
    188639pBMN HA-ATG13(W50D)For expression of human ATG13(W50D)

    Antibodies from Article