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Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation.

Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. Epub 2023 Feb 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188073pCAG-GST-ATG9 (828-839)-ATG101 (1-198)To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells
188074pCAG-ATG13 (1-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
188075pCAG-OSF-ATG13 (2-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
188076pCAG-MBP-ATG9Recombinant overexpression of human ATG9 in mammalian cells
188077pCAG-MBP-ATG9-V839AExpresses human ATG9-V839A mutant in mammalian cells
188078pCAG-MBP-ATG9-K838AExpresses human ATG9-K838A mutant in mammalian cells
188079pCAG-MBP-ATG9-H837AExpresses human ATG9-H837A mutant in mammalian cells
188080pCAG-MBP-ATG9-V836AExpresses human ATG9-V836A mutant in mammalian cells
188081pCAG-MBP-ATG9-Q835AExpresses human ATG9-Q835A mutant in mammalian cells
188082pCAG-MBP-ATG9-P834AExpresses human ATG9-P834A mutant in mammalian cells
188083pCAG-MBP-ATG9-P833AExpresses human ATG9-P833A mutant in mammalian cells
188084pCAG-MBP-ATG9-L832AExpresses human ATG9-L832A mutant in mammalian cells
188085pCAG-MBP-ATG9-D830A/E831AExpresses human ATG9-D830A/E831A mutant in mammalian cells
188086pCAG-MBP-ATG9 (1-830)Expresses human ATG9 C-terminal truncation mutant in mammalian cells
188087pCAG-MBP-Foldon-ATG9 (692-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
188088pCAG-MBP-Foldon-ATG9 (723-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
188089pCAG-MBP-Foldon-ATG9 (801-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
188090pCAG-MBP-Foldon-ATG9 (828-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
188091pCAG-MBP-Foldon-ATG9 (830-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
188092pCAG-MBP-ATG9 (692-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
188093pCAG-MBP-ATG9 (801-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
188094pCAG-OSF-ATG13 (2-197)-K15DExpresses human ATG13 HORMA K15D mutant in mammalian cells
188095pCAG-OSF-ATG13 (2-197)-F16DExpresses human ATG13 HORMA F16D mutant in mammalian cells
188096pCAG-OSF-ATG13 (2-197)-K18DExpresses human ATG13 HORMA K18D mutant in mammalian cells
188097pCAG-OSF-ATG13 (2-197)-F19DExpresses human ATG13 HORMA F19D mutant in mammalian cells
188098pCAG-OSF-ATG13 (2-197)-W50DExpresses human ATG13 HORMA W50D mutant in mammalian cells
188099pCAG-OSF-ATG13 (2-197)-E83LExpresses human ATG13 HORMA E83L mutant in mammalian cells
188100pCAG-OSF-ATG13 (2-197)-Y115DExpresses human ATG13 HORMA Y115D mutant in mammalian cells
188101pCAG-OSF-ATG13 (2-197)-Y118DExpresses human ATG13 HORMA Y118D mutant in mammalian cells
188102pCAG-OSF-ATG13 (2-197)-L151DExpresses human ATG13 HORMA L151D mutant in mammalian cells
188103pCAG-OSF-ATG13 (2-197)-Y152DExpresses human ATG13 HORMA Y152D mutant in mammalian cells
188104pCAG-OSF-ATG13 (2-197)-R153DExpresses human ATG13 HORMA R153D mutant in mammalian cells
188105pCAG-GST-ATG101-Y45DExpresses human ATG101 HORMA Y45D mutant in mammalian cells
188106pCAG-GST-ATG101-W110D/F112DExpresses human ATG101 HORMA W110D/F112D mutant in mammalian cells

Antibodies from Article