CREEPY: CRISPR-mediated editing of synthetic episomes in yeast.
Zhao Y, Coelho C, Lauer S, Majewski M, Laurent JM, Brosh R, Boeke JD
Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187970 | pYZ462 | TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t |
| 187971 | pYZ463 | TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t |
| 187972 | pYZ960 | TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3 |
| 192603 | pYZ959 | TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3 |
| 200227 | pYZ038 | universal template to build tRNA-gRNA array for multiplex editing CREEPY |