A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo.
Seo D, Koh B, Eom GE, Kim HW, Kim S
Nucleic Acids Res. 2023 Apr 18:gkad266. doi: 10.1093/nar/gkad266.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187620 | pDae029 (eMutaT7[TadA-8e]) | Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli |
| 187621 | pDae069 (eMutaT7[PmCDA1] with optimized RBS) | Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli |
| 187622 | pDae079 (eMutaT7[transition]) | Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli |