Live Cell Detection of Poly(ADP-Ribose) for Use in Genetic and Genotoxic Compound Screens.
Koczor CA, Haider AJ, Saville KM, Li J, Andrews JF, Beiser AV, Sobol RW
Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 10.3390/cancers14153676.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187609 | pLV-EF1A-LivePAR(R163A)-Hygro | EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette. |
| 187611 | pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro | Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette |
| 187612 | pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro | Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette |