Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing.
Volke DC, Martino RA, Kozaeva E, Smania AM, Nikel PI
Nat Commun. 2022 May 31;13(1):3026. doi: 10.1038/s41467-022-30780-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187596 | pMBEC2 | Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance |
| 187598 | pMBEC6 | Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance |
| 187599 | pMBEC8 | Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance |
| 187600 | pEX128·gRNA | Template for amplification of gRNA with Cas6 recognition site |