Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana.

Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N
Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
160595	pUPD2_miniDFR (GB2566)	Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region
187560	pEPKKα1KN0253	Transcriptional unit for constitutive expression of CUP2:GAL4 driven by the nos promoter
187561	pEPCTΩSP0021	Module for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2
187562	pEPCTΩSP0022	Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2
187563	pEPCTαKN0009	Transcriptional unit for copper-inducible expression of firefly luciferase driven by a minimal synthetic promoter with binding sites for CUP2
187564	pEPCTαKN0013	Transcriptional unit for copper-inducible expression of nanoluc luciferase driven by a minimal synthetic promoter with binding sites for CUP2
187565	pEPCTΩSP0017	Module for constitutive expression of firefly luciferase and nanoluc luciferase driven by 35s promoters
187566	pEPCTΩSP0018	Module for constitutive expression of nanoluc luciferase and firefly luciferase driven by 35s promoters
187568	pEPCTαKN0001	Transcriptional unit for constitutive expression of firefly luciferase driven by the 35s promoter
187569	pEPCTαKN0005	Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter
187570	GB UA_114 A	Transcriptional unit for constitutive expression of Gal4:ΦC31 driven by the 35s promoter
187571	pEPKKα1RKN0115	Transcriptional unit for constitutive expression of a TALE driven by the nos promoter
187605	pEPKKΩ1SP0678	Module for copper inducible expression of AtrD11, HarFAR and EaDAct
187803	GB2513	Module for constitutive expression of dCas9:EDLL, Ms2:VPR and a gRNA targeting the DFR promoter.
187804	GB1024	Module for the constitutive expression of the delta-11-desaturase from Amyelois transitella and a fatty acid reductase from Helicoverpa armigera.
187805	GB1022	Transcriptional unit for expression of diacylglycerol acetyltransferase from Euonymus alatus.
187806	GB3681	Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces cerevisiae S288C, codon optimized for Nicotiana.
187807	GB3682	Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces pastorianus strain CBS 1483 chromosome SeVIII-SeXV, codon optimized for Nicotiana.
187808	GB3683	Transcriptional unit for expression of 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus fortunei, codon optimized for Nicotiana.
187809	GB3897	Module for dCasEV2.1 activated expression of AtrD11, HarFAR and ScATF1 plus gRNA-1DFR.
187810	GB4068	Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
187811	GB4070	Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
187812	GB3898	Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and ScATF1.
187813	GB4356	Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and SpATF1-2.
187814	GB4360	Module for dCasEV2.1 activated expression ATF1, Atr delta11 and HarFAR.
187815	GB4361	Module for dCasEV2.1 activated expression of ATF1-2, HarFAR and Atr delta11.
187816	GB4366	Module for dCasEV2.1 activated expression of HarFAR, ATF1 and Atr delta11.
187817	GB4367	Module for dCasEV2.1 activated expression for HarFAR, ATF1-2 and Atr delta11.