Base editing in human cells with monomeric DddA-TALE fusion deaminases.
Mok YG, Lee JM, Chung E, Lee J, Lim K, Cho SI, Kim JS
Nat Commun. 2022 Jul 12;13(1):4038. doi: 10.1038/s41467-022-31745-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187409 | pCMV-AAAAA-nCas9(D10A)-UGI | DddA AAAAA variant |
| 187410 | pCMV-E1347A-nCas9(D10A)-UGI | DddA E1347A variant |
| 187411 | pCMV-UGI-nCas9(H840A)-GSVG | DddA GSVG variant |
| 187412 | pCMV-ND1 Left -GSVG-UGI | human mitochondrial DNA ND1 target TALE |
| 187413 | pCMV-ND1 Right-GSVG-UGI | human mitochondrial DNA ND1 target TALE |
| 187414 | pAAV-ND4 Right-GSVG-UGI | human mitochondrial DNA ND4 target TALE |
| 187415 | pAAV-ND4 Right-E1347A-UGI | human mitochondrial DNA ND4 target TALE |