Structure of a fully assembled tumor-specific T cell receptor ligated by pMHC.
Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM, Fernandes RA, Hummer G, Tampe R, Davis SJ
Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 10.1016/j.cell.2022.07.010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187351 | pHRSin_hTCRαγεζ | Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta) |
| 187352 | pHRSin_hTCRαγε | Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, and epsilon) |
| 187353 | pHRSin_hTCRβεζ | Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta) |
| 187354 | pHRSin_hTCRβε | Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 epsilon |
| 187355 | pHRSin_hCD3δ-GFP2 | Lentiviral expression of truncated hCD3 delta-GFP2 |
| 187356 | pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag | Bacterial expression and refolding of gp100 pMHC |
| 187357 | pET28a(+)_beta2M (MHC Class I light) | Bacterial expression and refolding of gp100 pMHC |
| 187358 | pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS | Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag |
| 187359 | pHRSin-IRES2-EmGFP_HPC-4 Ab light | Lentiviral expression of HPC-4 antibody light chain |
| 187360 | pHRSin_hTCRα | Lentiviral expression of GPa3b17 hTCR alpha |
| 187361 | pHRSin_hTCRβ | Lentiviral expression of GPa3b17 hTCR beta |
| 187440 | pMDG | VSV-G envelope expressing plasmid |
| 187441 | p8.91 | Lentiviral packaging plasmid expressing gag and pol. |
| 187572 | pHRSin_1G4 TCRα | Lentiviral expression TCR alpha(1G4) |
| 187573 | pHRSin_1G4 hTCRβ-SNAP | Lentiviral expression of hTCR beta(1G4), and SNAP |
| 187601 | pHRi_GPa3b17 TCRα | Lentiviral expression of hTCR alpha (GPa3b17) for low expression |
| 187602 | pHRi_GPa3b17 TCRβ | Lentiviral expression of hTCR beta (GPa3b17) for low expression |
| 187603 | pHRi_1G4 hTCRα | Lentiviral expression of hTCR alpha (1G4) for low expression |
| 187604 | pHRi_1G4 hTCRβ-SNAP | Lentiviral expression of hTCR beta(1G4), and SNAP for low expression |