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Tyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1.

Liang X, Budnar S, Gupta S, Verma S, Han SP, Hill MM, Daly RJ, Parton RG, Hamilton NA, Gomez GA, Yap AS
Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
187263pLL5.0 hCortKD-Flcortmut3YF EGFPLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant
187264pLL5.0 hCortKD-Flcortmut3YD EGFPLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant
187265pLL5.0 hCortKD-Flcortmut3YF mCherryLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant
187266pLL5.0 mCherry-hCortKD-Flcortmut3YD Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant
187267pLL5.0 hCortKD EGFPLentiviral expression of shRNA targeting Cortactin
187268pLL5.0 CortW22A mCherryLenticiral expression of mCherry-Cortactin W22A
187269pEGFP-SRGAP1 Expression of SRGAP1-EGFP

Antibodies from Article