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Recording gene expression order in DNA by CRISPR addition of retron barcodes.

Bhattarai-Kline S, Lear SK, Fishman CB, Lopez SC, Lockshin ER, Schubert MG, Nivala J, Church GM, Shipman SL
Nature. 2022 Aug;608(7921):217-225. doi: 10.1038/s41586-022-04994-6. Epub 2022 Jul 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
187201pSLS.400Expresses retron Eco4 RT
187202pSLS.402Expresses retron Eco1 RT
187203pSLS.405Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2
187204pSLS.407Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2
187205pSLS.408Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2
187207pSLS.416Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2
187208pSLS.419Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2
187209pSBK.009Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2
187210pSBK.010Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2
187211pSBK.011Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG]), and Cas1 + Cas2
187212pSBK.012Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]), and Cas1 + Cas2
187213pSBK.013Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2
187215pSBK.014Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2
187216pSBK.015Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC]), and Cas1 + Cas2
187217pSBK.016Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCT-TGC]), and Cas1 + Cas2
187218pSBK.079Expresses Eco1 RT and Cas1 + Cas2
187219pSBK.134Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively
187220pSBK.136Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively

Antibodies from Article