Analysis of the assembly pathway for membrane subunits of Complex I reveals that subunit L (ND5) can assemble last in E. coli.
Zhang F, Vik SB
BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2021.100027. Epub 2021 Oct 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187084 | pBAD33(A-N) | Medium expression of Complex I with subunit N-HA tagged |
| 187086 | pBAD33(A-H*HA-N) | Medium expression of Complex I with subunits N and H -HA tagged |
| 187087 | pBAD33(EFG) | Medium expression of subunits EFG |