Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation.
Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, Zarse K, Ewald CY, Ristow M
J Biol Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177783 | L4440_BioBrick-sgRNA | Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans |
| 177784 | L4440_BioBrick-hif-1-sgRNA-A | Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter) |
| 177785 | L4440_BioBrick-hif-1-sgRNA-B | Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter) |
| 177786 | L4440_BioBrick-hsf-1-sgRNA-A | Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter) |
| 177787 | L4440_BioBrick-hsf-1-sgRNA-B | Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter) |
| 177788 | pdestMB14_sur5p-dCas9-VP64 | Overexpression of dCas9-VP64 in C. elegans |
| 177811 | L4440_BioBrick-SCR-sgRNA-A | Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences) |
| 177812 | L4440_BioBrick-SCR-sgRNA-B | Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences) |