Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex.
Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H
Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186980 | pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya | mammalian expression of Cluc-Gluc reporter |
| 186981 | pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold | mammalian expression of Cas7-11 crRNA guide |
| 186982 | pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122 | mammalian expression of cas7-11-r1045-gggs-r1122 |
| 186983 | pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221 | mammalian expression of cas7-11-s1006-gggs-d1221 |
| 186984 | pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294 | mammalian expression of cas7-11-r978-gggs-r1294 |
| 186985 | pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc | AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide |
| 186987 | pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc | AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide |
| 186991 | pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide | AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide |
| 186993 | pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide | AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide |