Base editor scanning charts the DNMT3A activity landscape.

Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 10.1038/s41589-022-01167-4. Epub 2022 Oct 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
186970	pET28b-MBP-TEV-PWWP_DNMT3A	Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.
186969	pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40	PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.
186968	pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40	Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
186967	pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40	Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.
186966	pRRL-5xTetO-pEF-H2B-Citrine-SV40	Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.