Base editor scanning charts the DNMT3A activity landscape.
Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 10.1038/s41589-022-01167-4. Epub 2022 Oct 20.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186966 | pRRL-5xTetO-pEF-H2B-Citrine-SV40 | Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells. |
| 186967 | pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40 | Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells. |
| 186968 | pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40 | Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells. |
| 186969 | pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40 | PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells. |
| 186970 | pET28b-MBP-TEV-PWWP_DNMT3A | Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site. |