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The mIAA7 degron improves auxin-mediated degradation in C. elegans.

Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, Boxem M
G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 10.1093/g3journal/jkac222. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186950pJW234130aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
188324pJJS001PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.
188325pJJS002PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.
188326pJJS003PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.
188327pJJS004PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.
188328pJJS005PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
188329pJJS006PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
188330pJJS007PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
188331pJJS008PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
188332pJJS009PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.
188333pJJS010PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.
188334pJJS011PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.
188335pJJS012PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Antibodies from Article