Engineering Functional Membrane-Membrane Interfaces by InterSpy.
Moghimianavval H, Patel C, Mohapatra S, Hwang SW, Kayikcioglu T, Bashirzadeh Y, Liu AP, Ha T
Small. 2022 May 26:e2202104. doi: 10.1002/smll.202202104.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186900 | InterCatch-GFP | Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10 |
| 186901 | InterTag-BFP | Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag |
| 186902 | sCatch-GFP | Cell-free expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 |
| 186903 | sTag-BFP | Cell-free expression of soluble protein tagBFP-sfCherry11-SpyTag |
| 186904 | sCatch-GFP-6xHIS | Bacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tag |
| 186905 | sTag-BFP-6xHIS | Bacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tag |
| 186906 | InterCatch | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance gene |
| 186907 | InterCatch-1xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene |
| 186908 | InterCatch-2xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance gene |
| 186909 | InterCatch-3xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene |
| 186910 | InterTag | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance gene |
| 186911 | InterTag-1xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene |
| 186912 | InterTag-2xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance gene |
| 186913 | InterTag-3xL | Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene |
| 186914 | InterTag-3xL-Δtag | Sleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance gene |