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Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins.

Nelson SL, Li Y, Chen Y, Deshmukh L
J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186802Alpha-Synuclein-HisCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.
186803Twin-Strep(R)-Tev-GSGS-UbiquitinCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.
186804His-Tev-UbE1Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.
186805His-Tev-UbE2D3Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.
186806His-Tev-Nedd4LCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.
186807His-Tev-WWP2Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.
186808ALIX-His(1-868, P801G)Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).
189819GB1-His-Tev-ALIX-V (348-702)Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

Antibodies from Article