Biosensor reveals multiple sources for mitochondrial NAD(+).
Cambronne XA, Stewart ML, Kim D, Jones-Brunette AM, Morgan RK, Farrens DL, Cohen MS, Goodman RH
Science. 2016 Jun 17;352(6292):1474-7. doi: 10.1126/science.aad5168.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186787 | cytoplasmic NAD+ biosensor | NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm |
| 186788 | cytoplasmic cpVenus control | Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase |
| 186789 | nuclear NAD+ biosensor | NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus |
| 186790 | nuclear cpVenus control | Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase |
| 186791 | mito NAD+ biosensor | NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria |
| 186792 | mito cpVenus control | Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase |