Boosting targeted genome editing using the hei-tag.
Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, Medert R, Welz B, Freichel M, Wittbrodt J
Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186929 | pCS2+ heiCas9 | For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS |