Light-driven hydroxylation of testosterone by Synechocystis sp. PCC 6803 expressing the heterologous CYP450 monooxygenase CYP110D1
Mascia F, Pereira SB, Pacheco CC, Oliveira P, Solarczek J, Schallmey A, Kourist R, Alphand V and Tamagnini P.
Green Chemistry, 24 (2022):6156
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186707 | pSEVA251::PpsbA2*::B0032::CYP110D1 | CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis. |
| 186708 | pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag | N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis. |
| 186709 | pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 | CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis. |
| 186710 | pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag | N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis. |