Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells).

Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, Elchert AR, Benner L, Sobti S, Katz EY, Haase AD
Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
186650	puc19_N_tag	Empty donor plasmid.
186651	Donor_N_FH_Piwi	Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.
186652	Cas9_nonPuro_OSS	Cas9 plasmid to express the Cas9 in OSS cells
186653	piwi_sgRNA	piwi sgRNA plasmid
186655	Donor_N_FH_Shu	Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.
186656	Shu_sgRNA	shu sgRNA plasmid
186657	pDonor_N_FH_GLE	Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.
186658	GLE_sgRNA	GLE sgRNA plasmid
186659	PUC19_C_tag	Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.
186660	Donor_C_FH_VIR	Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.
186661	VIR_sgRNA	VIR sgRNA plasmid
186662	Donor_C_FH_Nbr	Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.
186663	Nbr_C_sgRNA1	Nbr C-tag sgRNA1 plasmid
186664	Nbr_C_sgRNA2	Nbr C-tag sgRNA2 plasmid
186667	Donor_ANKRD1_KO_Puro	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.
186668	Donor_ANKRD1_KO_Hygro	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.
186669	Cas9_ANKRD1_sgRNA2	Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.
186670	Donor_YTHDF2_KO_Puro	Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.
186671	Donor_YTHDF2_KO_Hygro	Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.
186672	Donor_YTHDF2_KO_Neo	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.
186673	Cas9_YTHDF2_sgRNA	YTHDF2 sgRNA plasmid
186674	pUC19_Ntag_BbsI-Mammalian	N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.