Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells).

Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, Elchert AR, Benner L, Sobti S, Katz EY, Haase AD
Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
186674	pUC19_Ntag_BbsI-Mammalian	N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.
186673	Cas9_YTHDF2_sgRNA	YTHDF2 sgRNA plasmid
186672	Donor_YTHDF2_KO_Neo	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.
186671	Donor_YTHDF2_KO_Hygro	Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.
186670	Donor_YTHDF2_KO_Puro	Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.
186669	Cas9_ANKRD1_sgRNA2	Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.
186668	Donor_ANKRD1_KO_Hygro	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.
186667	Donor_ANKRD1_KO_Puro	Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.
186664	Nbr_C_sgRNA2	Nbr C-tag sgRNA2 plasmid
186663	Nbr_C_sgRNA1	Nbr C-tag sgRNA1 plasmid