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Orthogonal Regulatory Circuits for Escherichia coli Based on the gamma-Butyrolactone System of Streptomyces coelicolor.

Biarnes-Carrera M, Lee CK, Nihira T, Breitling R, Takano E
ACS Synth Biol. 2018 Apr 20;7(4):1043-1055. doi: 10.1021/acssynbio.7b00425. Epub 2018 Mar 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186628pTE1059Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.
186629pTE1069SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Antibodies from Article