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The ER ladder is a unique morphological feature of developing mammalian axons.

Zamponi E, Meehl JB, Voeltz GK
Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 10.1016/j.devcel.2022.05.002. Epub 2022 May 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186597pLV-GFP-Sec61βThird generation lentiviral vector expressing GFP-Sec61β
186598Rtn2b-mNeonEncodes human full-length Reticulon 2 isoform B fused to mNeonGreen
186599Rtn2b-GFPEncodes human full-length Reticulon 2 isoform B fused to GFP
186600Rtn2b-mCherryEncodes human full-length Reticulon 2 isoform B fused to mCherry
186601Rtn2b-NTD1-271-mNeonEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen
186602Rtn2b-NTD1-135-mNeonEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen
186603Rtn2b-NTD1-60-mNeonEncodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen
186604Rtn2b-NTD136-271-mNeonEncodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen
186605Rtn2b-RHD272-472-mNeonEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen
186606Rtn2b-RHD272-472-GFPEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP
186607pLV-Rtn2b-R60f-mCherryThird generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry
186608Rtn2b-R60f-BFPEncodes human Reticulon 2 disease mutant R60f fused to BFP
186609Rtn4a-NTD1-135-mNeonEncodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreen
186610pLV-mNeon-TurboIDThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboID
186612pLV-Rtn2b-mNeon-TurboIDThird generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID
186613pLV-Kif5a-MycThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag
186614pLV-Kif5b-MycThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag
186615pLV-Kif5b-T92N-MycThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag
186616pLV-Kif5c-MycThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag
186617Klc1-GFPEncodes Kinesin Light Chain 1 fused to GFP
186618NpY-mNeonEncodes Neuropeptide Y fused to mNeonGreen
186619mNeon-Vamp2Encodes Vamp 2 fused to mNeonGreen
186620pLV-mCherry-Stim1Third generation lentiviral vector expressing Stim1-wt fused to mCherry
186621YFP-Stim1-ΔCTDEncodes Stim1 with C-terminal domain deletion fused to YFP
186622YFP-Stim1-D76AEncodes Stim1 mutant D76A fused to YFP

Antibodies from Article