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Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21.

Lozano Terol G, Gallego-Jara J, Sola Martinez RA, Martinez Vivancos A, Canovas Diaz M, de Diego Puente T
Front Microbiol. 2021 Jun 21;12:682001. doi: 10.3389/fmicb.2021.682001. eCollection 2021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186563pSF-p15A-YFPp15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186564pSF-pMB1'-t7- YFPpMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186565pSF-pMB1'- trc-YFPpMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186566pSF-pMB1'- tac-YFPpMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186567pSF-pMB1'- BAD-YFPpMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186568pSF-p15A-T7- YFPp15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186569pSF-p15A-trc- YFPp15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186570pSF-p15A-tac- YFPp15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression
186571pSF-p15ABAD- YFPp15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Antibodies from Article