Genome-wide protein-DNA interaction site mapping in bacteria using a double-stranded DNA-specific cytosine deaminase.
Gallagher LA, Velazquez E, Peterson SB, Charity JC, Radey MC, Gebhardt MJ, Hsu F, Shull LM, Cutler KJ, Macareno K, de Moraes MH, Penewit KM, Kim J, Andrade PA, LaFramboise T, Salipante SJ, Reniere ML, de Lorenzo V, Wiggins PA, Dove SL, Mougous JD
Nat Microbiol. 2022 Jun;7(6):844-855. doi: 10.1038/s41564-022-01133-9. Epub 2022 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186556 | pPSV39-ugi | Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI) |
| 186557 | pPSV39-dddAI | Broad host range regulated expression of the DddA immunity determinant (DddAI) |
| 186558 | pPSV39-dddAI-FLAG | Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG) |
| 186559 | pMMB67EH-dddAI | Broad host range regulated expression of the DddA immunity determinant (DddAI) |
| 186560 | pMMB67EH-dddAI-FLAG | Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG) |
| 186561 | pBS10-riboE-dddAI | Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes |
| 186562 | pBS10-ribE-dddAI-FLAG | Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG) |