Chemically stable fluorescent proteins for advanced microscopy.
Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF
Nat Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 10.1038/s41592-022-01660-7.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186515 | Tubulin-hfYFP | Localization of hyperfolder YFP fluorescent protein to tubulin |
| 186518 | ER2-hfYFP | Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency |
| 186520 | pCytERM-hfYFP | Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay) |
| 186522 | pBAD-hfYFP | Hyperfolder YFP fluorescent protein: bacterial expression plasmid |
| 186523 | pcDNA3.1-hfYFP | Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells |
| 186524 | LifeAct-mhYFP | Localization of monomeric hyperfolder YFP fluorescent protein to actin |
| 186525 | Tubulin-mhYFP | Localization of monomeric hyperfolder YFP fluorescent protein to tubulin |
| 186526 | Mito-mhYFP | Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria |
| 186527 | Clathrin-mhYFP | Localization of monomeric hyperfolder YFP fluorescent protein to clathrin |
| 186528 | ER2-mhYFP | Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency |
| 186530 | pCytERM-mhYFP | Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay) |
| 186532 | pBAD-mhYFP | Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid |
| 186533 | pcDNA3.1-mhYFP | Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells |
| 186534 | LifeAct-LSSmGFP | Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin |
| 186535 | Tubulin-LSSmGFP | Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin |
| 186536 | Mito-LSSmGFP | Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria |
| 186537 | Clathrin-LSSmGFP | Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to clathrin |
| 186538 | ER2-LSSmGFP | Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency |
| 186539 | H2B-LSSmGFP | Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B |
| 186540 | pCytERM-LSSmGFP | Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay. |
| 186542 | pBAD-LSSmGFP | LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid |
| 186543 | pcDNA3.1-LSSmGFP | LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells |
| 186544 | pcDNA3.1-3xhfYFP | Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells |
| 186545 | pcDNA3.1-LSSA12 | LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells |
| 186546 | pBAD-LSSA12 | LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid |
| 186547 | pBAD-FOLD6 | FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid |
| 186548 | ER2-mGreenLantern | Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency |
| 191195 | pET28a-hfYFP-TEV-Bcx | Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification |
| 191196 | pET28a-hfYFP-TEV-mScarlet-i | Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification |
| 191197 | pET28a-hfYFP-TEV-SAV | Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification |
| 191199 | pET28a-LSSmGFP-TEV-mScarlet-i | Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification |
| 191200 | pET28a-LSSmGFP-TEV-SAV | Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification |
| 191201 | pAAV-CAG-hfYFP | Constitutive CAG-driven expression of hfYFP in animals using AAV |