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Chemically stable fluorescent proteins for advanced microscopy.

Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF
Nat Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 10.1038/s41592-022-01660-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186515Tubulin-hfYFPLocalization of hyperfolder YFP fluorescent protein to tubulin
186518ER2-hfYFPLocalization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
186520pCytERM-hfYFPVisualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)
186522pBAD-hfYFPHyperfolder YFP fluorescent protein: bacterial expression plasmid
186523pcDNA3.1-hfYFPHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
186524LifeAct-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to actin
186525Tubulin-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to tubulin
186526Mito-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to mitochondria
186527Clathrin-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to clathrin
186528ER2-mhYFPLocalization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
186530pCytERM-mhYFPVisualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
186532pBAD-mhYFPMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid
186533pcDNA3.1-mhYFPMonomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
186534LifeAct-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin
186535Tubulin-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin
186536Mito-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria
186537Clathrin-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to clathrin
186538ER2-LSSmGFPLocalization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency
186539H2B-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
186540pCytERM-LSSmGFPVisualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.
186542pBAD-LSSmGFPLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid
186543pcDNA3.1-LSSmGFPLSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells
186544pcDNA3.1-3xhfYFPHyperfolder YFP, triple fusion: cytosolic expression in mammalian cells
186545pcDNA3.1-LSSA12LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells
186546pBAD-LSSA12LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid
186547pBAD-FOLD6FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid
186548ER2-mGreenLanternLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency
191201pAAV-CAG-hfYFPConstitutive CAG-driven expression of hfYFP in animals using AAV

Antibodies from Article