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Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites.

Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nomine Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F
EMBO Rep. 2018 Jul;19(7). pii: embr.201745453. doi: 10.15252/embr.201745453. Epub 2018 Jun 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186467pQCXIP GFP-MOSPD2 WTExpression of human MOSPD2 fused to EGFP in mammalian cells
186468pQCXIP GFP-MOSPD2 RD/LDExpression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells
186469pET15b MOSPD2 [282-490]Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria
186470pET15b MOSPD2 RD/LD [282-490]Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria
226407pmCherryC1 VAP-AExpression of human VAP-A fused to mCherry in mammalian cells
226408pmCherryC1 VAP-A KD/MDExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells
226409pmCherryC1 VAP-BExpression of human VAP-B fused to mCherry in mammalian cells
226410pmCherryC1 VAP-B KD/MDExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Antibodies from Article