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Roi Avraham Lab: Bacterial fluorescent reporters and tags toolkit

Avraham, Roi
Unpublished

Plasmids from Article

ID Plasmid Purpose
187378pFPV25.1_dGFPmut3-LVArpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)
187379pBAD_dGFPmut3-AAVarabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
187381R6K_tac_GFPmut3_ChIntchromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
187382R6K_tac_mCherry_ChIntchromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry
187385pKD3_mCherryTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
187386pKD4_mCherryTo chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT
187387pBAD_mCherryarabinose-inducible expression of mCherry
187388R6K_tac_dGFPmut3_ChIntchromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
187389pBAD_mCherry_GFP_++arabinose-inducible expression of GFP and mCherry
187390pBAD_mCherry_GFP_-+arabinose-inducible expression of GFP and mCherry
187391pBAD_GFP_mCherry_++arabinose-inducible expression of GFP and mCherry
187392pBAD_GFP_mCherry_-+arabinose-inducible expression of GFP and mCherry
187393R6K_BAD_GFP-mCherry_ChInt++chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
187394R6K_BAD_GFP-mCherry_ChInt--chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
187396R6K_BAD_mCherry-GFP_ChInt--chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry

Antibodies from Article