Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.
Kobercova E, Srba M, Fischer L
Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186423 | pUPD2_SulR(CDS) | provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5) |
| 186424 | pDGB1alpha11_SulR | transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly |
| 186425 | pDGB1alpha2_SulR | transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter |
| 186426 | pDGB3omega1_KanR_BastaR | optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) |
| 186427 | pDGB1alpha2_KanR_BastaR_Rb7_GFP | optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP) |