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Patrick Lemaire Lab: Lemaire Lab Plasmids 2022

Unpublished

Plasmids from Article

ID Plasmid Purpose
186353pENT L1-Kozak-ERK-KTR-L2Entry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
186354pENT-L1-Kozak-PH-domain-L2Entry clone with ORF encoding PH domain of human phospholipase C delta 1 flanked by Gateway recombination sequences
186375pDEST-Spe4-RfADestination vector for the expression of a protein of interest into embryos, by mRNA microinjection
186376pDEST-Spe4-Kozak-emiRFP703-RfADestination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection
186377pDEST-Spe4-Kozak-Halotag-RfADestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
186378pDEST-Spe4-Kozak-mCherry-RfADestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjection
186379pDEST-Spe4-Kozak-mClover3-RfADestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjection
186380pDEST-Spe4-Kozak-mEos4b-RfADestination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjection
186381pDEST-Spe4-Kozak-miRFP713-RfADestination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
186382pDEST-Spe4-Kozak-mNeonGreen-RfADestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjection
186383pDEST-Spe4-Kozak-mScarlet-I-RfADestination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjection
186384pDEST-Spe4-Kozak-mVenus-RfADestination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjection
186385pDEST-Spe4-Kozak-Snaptag-RfADestination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection
186386pDEST-Spe4-RfA-emiRFP703-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjection
186387pDEST-Spe4-RfA-Halotag-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
186388pDEST-Spe4-Rfa-mCherry-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjection
186389pDEST-Spe4-RfA-mClover3-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjection
186390pDEST-Spe4-RfA-mEos4b-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjection
186391pDEST-Spe4-RfA-miRFP713-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection
186392pDEST-Spe4-RfA-mNeonGreen-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjection
186393pDEST-Spe4-RfA-mScarlet-I-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
186394pDEST-Spe4-RfA-mVenus-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection
186395pDEST-Spe4-RfA-Snaptag-stopDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection

Antibodies from Article