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Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes.

Vicencio J, Sanchez-Bolanos C, Moreno-Sanchez I, Brena D, Vejnar CE, Kukhtar D, Ruiz-Lopez M, Cots-Ponjoan M, Rubio A, Melero NR, Crespo-Cuadrado J, Carolis C, Perez-Pulido AJ, Giraldez AJ, Kleinstiver BP, Ceron J, Moreno-Mateos MA
Nat Commun. 2022 May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
179316pT3TS-zSpGIn vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter
179317pT3TS-zSpRYIn vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
179318pET28b-SpG-HisPlasmid for bacterial expression and purification of SpG-Cas9
179320pET28b-SpRY-HisPlasmid for bacterial expression and purification of SpRY-Cas9

Antibodies from Article