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helixCAM: A platform for programmable cellular assembly in bacteria and human cells.

Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM
Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186303PB_TRE_Z17-PDGFR_EF1a_mCherryPlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.
186304PB_TRE_Z18-PDGFR_EF1a_eGFPPlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.
186305PB_TRE_P3-PDGFR_EF1a_iRFP670Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.
186306PB_TRE_AP4-PDGFR_EF1a_eBFP2Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
186307PB_TRE_P9-PDGFR_EF1a_mPlumPlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.
186308PB_TRE_AP10-PDGFR_EF1a_mOrangePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction
186309PB_TRE_sg30-PDGFR_EF1a_mCherryPlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction
186310PB_TRE_sg61-PDGFR_EF1a_eGFPPlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction
186311PB_TRE_sg83-PDGFR_EF1a_iRFP670Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction
186312PB_TRE_sg88-PDGFR_EF1a_eBFP2Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
186313pQE80_T5_Z17-ehaA_LacIPlasmid for inducible E coli expression of the Z17 helixCAM.
186314pQE80_T5_Z18-ehaA_LacIPlasmid for inducible E coli expression of the Z18 helixCAM.
186315pQE80_T5_P3-ehaA_LacIPlasmid for inducible E coli expression of the P3 helixCAM.
186316pQE80_T5_AP4-ehaA_LacIPlasmid for inducible E coli expression of the AP4 helixCAM.
186317pQE80_T5_P9-ehaA_LacIPlasmid for inducible E coli expression of the P9 helixCAM.
186318pQE80_T5_AP10-ehaA_LacIPlasmid for inducible E coli expression of the AP10 helixCAM.

Antibodies from Article