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The ER membrane-bending protein RETICULON facilitates chloroplast relocation movement in Marchantia polymorpha.

Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama Y
Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186287pMpGWB302-er-mCherryBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)
186288pMpGWB402-er-mCherryBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)
186291pMpGE_En03-sgRNA-Target1 (MpRTN1)Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells
186295pMpGE010-sgRNA-Target1 (MpRTN1)Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)
228472pB4GWnY-MpPHOTVector for the expression of MpPHOT fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)
228473pB4GWcY-MpPHOTVector for the expression of MpPHOT fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)
228474pGWB602-MpPHOT-3xFLAGMpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)
228475pGWB605-MpRTN1Binary vector for the expression of MpRTN1 fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)
228476pMpGWB306-MpRTN1Binary vector for the expression of MpRTN1 in plants
228477pB4GWnY-MpRTN1Vector for the expression of MpRTN1 fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)
228478pB4GWcY-MpRTN1Vector for the expression of MpRTN1 fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Antibodies from Article