Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex.
Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA
Nat Commun. 2021 May 6;12(1):2571. doi: 10.1038/s41467-021-22900-y.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186274 | pET_His6-MBP-TEV-Cas6-RT-Cas1_WT | Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli |
| 186275 | pET_His6-MBP-TEV-Cas2 | Expresses Thiomicrospira Cas2 in E. coli |
| 186276 | pCRISPR | Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays |
| 186277 | pCRISPR_full-site | Reporter plasmid for full-site selection assay |