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A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.

May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ
Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185986N-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 Nucleoprotein fused to BioID2 and HA tag
185987E-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 Envelope protein fused to BioID2 and HA tag
185988M-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 Membrane protein fused to BioID2 and HA tag
185989ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 ORF3a fused to BioID2 and HA tag
185990ORF6-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 ORF6 fused to BioID2 and HA tag
185991ORF7a-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 ORF7a fused to BioID2 and HA tag
185992BioID2-1x-ORF7b pBabe-puro (SARS-CoV-2)Expresses BioID2 fused to SARS-CoV-2 ORF7b and myc tag
185993ORF8-1x-TurboID pbabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 ORF8 to TurboID and 3xHA tag
185994BioID2-1x-ORF9c pBabe-puro (SARS-CoV-2)Expresses myc-tagged BioID2 fused to SARS-CoV-2 ORF9c
185995ORF10-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 ORF10 fused to BioID2 and HA tag
185996NSP2-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP2 fused to BioID2 and HA tag
185997BioID2-1x-NSP3 pBabe-puro (SARS-CoV-2)Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP3
185998NSP4-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP4 fused to BioID2 and HA tag
185999NSP5-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP5 fused to BioID2 and HA tag
186000NSP6-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP6 fused to BioID2 and HA tag
186001NSP7-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP7 fused to BioID2 and HA tag
186002NSP8-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP8 fused to BioID2 and HA tag
186003NSP9-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP9 fused to BioID2 and HA tag
186004NSP10-1x-BioID2 pbabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP10 fused to BioID2 and HA tag
186005NSP11-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP11 fused to BioID2 and HA tag
186006BioID2-1x-NSP12 pBabe-puro (SARS-CoV-2)Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP12
186007NSP13-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP13 fused to BioID2 and HA tag
186008NSP14-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP14 fused to BioID2 and HA tag
186009NSP15-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP15 fused to BioID2 and HA tag
186010NSP16-1x-BioID2 pBabe-puro (SARS-CoV-2)Expresses SARS-CoV-2 NSP16 fused to BioID2 and HA tag
186011SS-3xHA-TurboID-KDEL pBabe-puroExpresses TurboID confined to ER by human albumin signal sequence (SS) and KDEL. 3xHA tag.

Antibodies from Article