Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases.

Huang YH, Trapp V, Puro O, Makinen JJ, Metsa-Ketela M, Wahl MC, Belogurov GA
Methods Enzymol. 2022;675:207-233. doi: 10.1016/bs.mie.2022.07.010. Epub 2022 Aug 19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
185985	pOP004	Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
186030	pOP005	Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter