A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.
Vides EG, Adhikari A, Chiang CY, Lis P, Purlyte E, Limouse C, Shumate JL, Spinola-Lasso E, Dhekne HS, Alessi DR, Pfeffer SR
Elife. 2022 Sep 23;11:e79771. doi: 10.7554/eLife.79771.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186012 | pCMV Flag-LRRK2 K17A/K18A | Flag-LRRK2 K17A/K18A |
| 186013 | pCMV Flag-LRRK2 K17A/K18A/R1441G | Flag-LRRK2 K17A/K18A/R1441G |
| 186014 | pET14b His Rab8a Q67L | His Rab8a Q67L |
| 186015 | pET21b GFP-Rab10 Q68LHis | GFP-Rab10 Q68LHis |
| 186016 | pQE-80L 2xHis-LRRK2 Armadillo 1-159 | 2xHis-LRRK2 Armadillo 1-159 |
| 186017 | pQE-80L 2xHis-LRRK2 Armadillo 1-552 | 2xHis-LRRK2 Armadillo 1-552 |
| 186018 | pQE-80L 2xHis-LRRK2 Armadillo 350-550 | 2xHis-LRRK2 Armadillo 350-550 |
| 186019 | pQE-80L 2xHis-LRRK2 Armadillo-K17A | 2xHis-LRRK2 Armadillo-K17A |
| 186020 | pQE-80L 2xHis-LRRK2 Armadillo- K18A | 2xHis-LRRK2 Armadillo- K18A |
| 186021 | pQE-80L 2xHis-Rab29 | 2xHis-Rab29 |