Force generation of KIF1C is impaired by pathogenic mutations.

Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A
Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 10.1016/j.cub.2022.07.029. Epub 2022 Aug 11. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
185978	pFastBac-M13-6His-KIF1C_P176L-GFP	Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.
185979	pFastBac-M13-6His-KIF1C_R169W-GFP	Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.
185980	pKIF1C_P176L-GFP	Mammalian expression of human KIF1C with P176L mutation and GFP tag.
185981	pKIF1C_R169W-GFP	Mammalian expression of human KIF1C with R169W mutation and GFP tag.
185982	pKIF1C-mCherry-FRB	Mammalian expression of human KIF1C with mCherry and FRB tags.
185983	pKIF1C_P176L-mCherry-FRB	Mammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.
185984	pKIF1C_R169W-mCherry-FRB	Mammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.