Processing two environmental chemical signals with a synthetic genetic IMPLY gate, a 2-input-2-output integrated logic circuit, and a process pipeline to optimize its systems chemistry in Escherichia coli.

Mukhopadhyay S, Sarkar K, Srivastava R, Pal A, Bagh S
Biotechnol Bioeng. 2020 May;117(5):1502-1512. doi: 10.1002/bit.27286. Epub 2020 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
185967	pNB_EA_3EGFP	Expresses EGFP from PR promoter in E.coli Dh5alpha cell
185968	pNB_EA_R1CiM_4EGFP_R3EGFPi2	Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
185969	pNB_EA_R3tdTomatoi2	Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.