Benjamin Kleinstiver Lab: ABE8e and ABE8.20m PAM variant constructs
Kleinstiver Lab
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185913 | pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309) | pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP |
| 185914 | pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999) | pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP |
| 185918 | pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166) | pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP |
| 185919 | pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164) | pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP |