Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae.
Peng B, Bandari NC, Lu Z, Howard CB, Scott C, Trau M, Dumsday G, Vickers CE
Commun Biol. 2022 Feb 16;5(1):135. doi: 10.1038/s42003-022-03070-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185837 | pAF4EH4R | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185839 | pAF7E1 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185840 | pILGFP4D42 | Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter |
| 185841 | pILGFP4D41 | Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter |
| 185842 | pILGFP9E3 | Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter |
| 185843 | pAF9C3 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185844 | pAF4E9D | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185845 | pAF9D91 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185846 | pAF9D92 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185847 | pAF9D93 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185848 | pAF9D94 (pAF10C6) | Expressing artificial trans-repressor in Saccharomyces cerevisiae |
| 185849 | pAFZifPZ431 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185850 | pAFZifPZ432 | Expressing artificial trans-activator in Saccharomyces cerevisiae |
| 185851 | pILGFP3A2 (1) | Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter |
| 185852 | pILGFP3A2 (2) | Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter |
| 185853 | pIRTetR-GAL80 | Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae |
| 185854 | pILGFP8EFA | Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter |
| 185855 | pILGFP10E1A | Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter |
| 185856 | pILGFP10E1B | Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter |
| 185857 | pILGFP10CF72 | Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter |
| 185858 | pILGFP10CF72 (B9) | Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter |
| 185859 | pILGFP10CF72 (B11) | Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter |
| 185860 | pILGFP10CF71 | Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter |