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Systematic profiling of conditional degron tag technologies for target validation studies.

Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, Ianari A
Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185760SFFV-DTAG-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185761SFFV-SMASH-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185762SFFV-HALO-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185763SFFV-AID-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185764SFFV-IKZF3-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185765SFFV-DTAG-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185766SFFV-SMASH-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185767SFFV-HALO-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185768SFFV-AID-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185769SFFV-IKZF3-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185770PGK-DTAG-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185771PGK-SMASH-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185772PGK-HALO-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185773PGK-AID-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185774PGK-IKZF3-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185775PGK-DTAG-NTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185776PGK-SMASH-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185777PGK-HALO-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185778PGK-AID-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
185779PGK-IKZF3-CTERM-GFPDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Antibodies from Article