Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9.
Akimoto G, Fernandes AP, Bode JW
ACS Cent Sci. 2022 Feb 23;8(2):275-281. doi: 10.1021/acscentsci.1c01490. Epub 2022 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185755 | pET-ChE1 v4.5 | Bacterial expression of ChE1 v4.5 |
| 185756 | pUb-HisUb-Ubc9-ChE1 v4.5 | Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5 |
| 185757 | pSub-MBP-GFP(2M-IKQE) | Bacterial expression of MBP-GFP(2M IKQE) |
| 185758 | pSub-Asp6-MBP-HRas | Bacterial expression of Asp6-MBP-HRas |
| 185759 | pET28-His6-Ubc9-K14R | Bacterial expression of His6-Ubc9-K14R |