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A vector system for fast-forward studies of the HOPZ-ACTIVATED RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana benthamiana.

Harant A, Pai H, Sakai T, Kamoun S, Adachi H
Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 10.1093/plphys/kiab471. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158479pZA1Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon
158480pZA2Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon
158481pZA3Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon
158482pZA4Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon
158483pZA5Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 with STOP codon
158484pZA6Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
158485pZA7Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
158486pZA8Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon
158487pZA9Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon
158488pZA10Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon
158489pZA11Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon
158490pZA12Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon
158491pZA13Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon
158492pZA14Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
158493pZA15Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
158494pZA16Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon
158495pZA17In planta gene expression of Golden gate compatible A. thaliana ZAR1. Km plant selection marker.
158496pZA18In planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.
158497pZA19In planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.
158498pZA20In planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.
158499pZA21In planta gene expression of Golden gate compatible N. benthamiana ZAR1. Km plant selection marker.
158500pZA22In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V. Km plant selection marker.
158501pZA23In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E. Km plant selection marker.
158502pZA24In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E. Km plant selection marker.
158503pZA25In planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.
158504pZA26In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.
158505pZA27In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.
158506pZA28In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.
158507pZA29In planta gene expression of Golden gate compatible N. benthamiana ZAR1-4xMyc. Km plant selection marker.
158508pZA30In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-4xMyc. Km plant selection marker.
158509pZA31In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.
158510pZA32In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-4xMyc. Km plant selection marker.
158511pZA33In planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.
158512pZA34In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.
158513pZA35In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.
158514pZA36In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.
158515pZA37In planta gene expression of Golden gate compatible N. benthamiana ZAR1-6xHA. Km plant selection marker.
158516pZA38In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-6xHA. Km plant selection marker.
158517pZA39In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-6xHA. Km plant selection marker.
158518pZA40In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-6xHA. Km plant selection marker.
158519pZA41In planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.
158520pZA42In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.
158521pZA43In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.
158522pZA44In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.
158523pZA45In planta gene expression of Golden gate compatible N. benthamiana ZAR1-eGFP. Km plant selection marker.
158524pZA46In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.
158525pZA47In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.
158526pZA48In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.
174762pZA49Entry clone for Golden Gate assembly
174763pZA50Entry clone for Golden Gate assembly
174764pZA51Entry clone for Golden Gate assembly
174765pZA52Entry clone for Golden Gate assembly
174766pZA53Entry clone for Golden Gate assembly
174767pZA54Entry clone for Golden Gate assembly
174768pZA55Entry clone for Golden Gate assembly
174769pZA56Entry clone for Golden Gate assembly
174770pZA57In planta gene expression
174771pZA58In planta gene expression
174772pZA59In planta gene expression
174773pZA60In planta gene expression
174774pZA61In planta gene expression
174775pZA62In planta gene expression
174776pZA63In planta gene expression
174777pZA64In planta gene expression
174778pZA65In planta gene expression
174779pZA66In planta gene expression
174780pZA67In planta gene expression
174781pZA68In planta gene expression
174782pZA69RNAi
174783pZA72In planta gene expression
174784pZA70RNAi
174785pZA71In planta gene expression

Antibodies from Article