A Markerless CRISPR-Mediated System for Genome Editing in Candida auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
Ennis CL, Hernday AD, Nobile CJ
Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 10.1128/Spectrum.01820-21. Epub 2021 Nov 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174405 | pCE27 | C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct. |
| 174409 | pCE35 | C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct. |
| 174432 | pCE38 | C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct. |
| 174433 | pCE41 | C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct. |
| 174434 | pCE1 | Candida optimized monomeric eGFP with RIPLING linker sequence. |