Identification of proximal SUMO-dependent interactors using SUMO-ID.
Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD
Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208035 | TRIPZ-CTID195-GSQ-SUMO1nc-PURO | Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin |
| 208037 | TRIPZ-CTID195-GSQ-UBnc-PURO | Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin |
| 208038 | Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST | Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin |
| 208039 | Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST | Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin |
| 208040 | Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST | Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin |
| 208041 | Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST | Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin |